Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus
Identifieur interne : 005B69 ( Main/Exploration ); précédent : 005B68; suivant : 005B70Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus
Auteurs : Burtram C. Fielding [Singapour] ; Yee-Joo Tan [Singapour] ; SHEN SHUO [Singapour] ; Timothy H. P. Tan [Singapour] ; Eng-Eong Ooi [Singapour] ; SENG GEE LIM [Singapour] ; WANJIN HONG [Singapour] ; Phuay-Yee Goh [Singapour]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Cadres ouverts de lecture (génétique), Cellules Vero, Données de séquences moléculaires, Humains, Protéines de la matrice virale, Protéines membranaires (), Protéines membranaires (génétique), Protéines membranaires (métabolisme), Protéines virales (), Protéines virales (génétique), Protéines virales (métabolisme), Réticulum endoplasmique (métabolisme), Signaux de triage des protéines, Spécificité d'espèce, Séquence d'acides aminés, Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Cadres ouverts de lecture, Protéines membranaires, Protéines virales, Virus du SRAS.
- métabolisme : Protéines membranaires, Protéines virales, Réticulum endoplasmique, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- Pascal (Inist)
- Animaux, Cellules Vero, Coronavirus, Caractérisation, Données de séquences moléculaires, Humains, Protéine A, Microbiologie, Protéines de la matrice virale, Protéines membranaires, Protéines virales, Signaux de triage des protéines, Spécificité d'espèce, Séquence d'acides aminés, Transfection, Virologie, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Characterization, Chlorocebus aethiops, Coronavirus, Endoplasmic Reticulum (metabolism), Humans, Membrane Proteins (chemistry), Membrane Proteins (genetics), Membrane Proteins (metabolism), Microbiology, Molecular Sequence Data, Open Reading Frames (genetics), Protein A, Protein Sorting Signals, SARS Virus (genetics), SARS Virus (metabolism), SARS Virus (pathogenicity), Severe acute respiratory syndrome, Species Specificity, Transfection, Vero Cells, Viral Matrix Proteins, Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (metabolism), Virology.
- MESH :
- chemical , chemistry : Membrane Proteins, Viral Proteins.
- chemical , genetics : Membrane Proteins, Viral Proteins.
- genetics : Open Reading Frames, SARS Virus.
- metabolism : Endoplasmic Reticulum, Membrane Proteins, SARS Virus, Viral Proteins.
- pathogenicity : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Humans, Molecular Sequence Data, Protein Sorting Signals, Species Specificity, Transfection, Vero Cells, Viral Matrix Proteins.
Abstract
A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoV-infected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.
Affiliations:
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Fielding</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author><author><name sortKey="Shen Shuo" sort="Shen Shuo" uniqKey="Shen Shuo" last="Shen Shuo">SHEN SHUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author><author><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng-Eong" last="Ooi">Eng-Eong Ooi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Environmental Health Institute. National Environmental Agency</s1><s2>Singapore 117610</s2><s3>SGP</s3><sZ>5 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Environmental Health Institute. National Environmental Agency</wicri:noRegion></affiliation></author><author><name sortKey="Seng Gee Lim" sort="Seng Gee Lim" uniqKey="Seng Gee Lim" last="Seng Gee Lim">SENG GEE LIM</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Medicine, National University Hospital</s1><s2>Singapore 119074</s2><s3>SGP</s3><sZ>6 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 119074</wicri:noRegion></affiliation></author><author><name sortKey="Wanjin Hong" sort="Wanjin Hong" uniqKey="Wanjin Hong" last="Wanjin Hong">WANJIN HONG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author><author><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore 117609</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 117609</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Characterization</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Endoplasmic Reticulum (metabolism)</term><term>Humans</term><term>Membrane Proteins (chemistry)</term><term>Membrane Proteins (genetics)</term><term>Membrane Proteins (metabolism)</term><term>Microbiology</term><term>Molecular Sequence Data</term><term>Open Reading Frames (genetics)</term><term>Protein A</term><term>Protein Sorting Signals</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (pathogenicity)</term><term>Severe acute respiratory syndrome</term><term>Species Specificity</term><term>Transfection</term><term>Vero Cells</term><term>Viral Matrix Proteins</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture (génétique)</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Protéines de la matrice virale</term><term>Protéines membranaires ()</term><term>Protéines membranaires (génétique)</term><term>Protéines membranaires (métabolisme)</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Réticulum endoplasmique (métabolisme)</term><term>Signaux de triage des protéines</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Transfection</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Protéines membranaires</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endoplasmic Reticulum</term><term>Membrane Proteins</term><term>SARS Virus</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines membranaires</term><term>Protéines virales</term><term>Réticulum endoplasmique</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Molecular Sequence Data</term><term>Protein Sorting Signals</term><term>Species Specificity</term><term>Transfection</term><term>Vero Cells</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus</term><term>Caractérisation</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Protéine A</term><term>Microbiologie</term><term>Protéines de la matrice virale</term><term>Protéines membranaires</term><term>Protéines virales</term><term>Signaux de triage des protéines</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Transfection</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoV-infected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.</div></front></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><country name="Singapour"><noRegion><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name></noRegion><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng-Eong" last="Ooi">Eng-Eong Ooi</name><name sortKey="Seng Gee Lim" sort="Seng Gee Lim" uniqKey="Seng Gee Lim" last="Seng Gee Lim">SENG GEE LIM</name><name sortKey="Seng Gee Lim" sort="Seng Gee Lim" uniqKey="Seng Gee Lim" last="Seng Gee Lim">SENG GEE LIM</name><name sortKey="Shen Shuo" sort="Shen Shuo" uniqKey="Shen Shuo" last="Shen Shuo">SHEN SHUO</name><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Wanjin Hong" sort="Wanjin Hong" uniqKey="Wanjin Hong" last="Wanjin Hong">WANJIN HONG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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